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How does it work?

Bacterial enzyme preparations are a mixture of selected natural bacterial cultures, enzymes and nutrients that enhance and accelerate nature's own biodegradation processes. In the natural environment, both bacteria and enzymes (these are the catalysts that decompose macromolecules) play an important role in the process of cleavage and decomposition of organic substances.

Bacteria produce enzymes that are indispensable for the metabolism of the nutrient source (in our case of organic waste) into energy necessary for the further growth of the living organisms. Enzymes are an instrument of that phase of metabolism in which complex compounds are cleaved to simpler ones. This cleavage accelerates the process of transforming the nutrient source into available and easily digestible batches for bacteria. In a relatively short time, after the contact of specially selected bacteria with an aqueous environment containing organic waste, spores of bacteria begin to transform from a dormant state into the active state, while producing specific enzymes and decomposing or eating the organic waste present in the source.

Applied microorganisms are capable of exponential growth (their number is doubled approximately every thirty minutes). The by-products of this bacterial enzyme activity are H2O and CO2.

Cellulase a complex of bio-enzymes decomposing cellulose.
Amylase a complex of bio-enzymes decomposing starchy organic compounds and hydrocarbons.
Protease a complex of bio-enzymes decomposing different proteins.
Lipase a complex of bio-enzymes decomposing fats and vegetable oils.
Urease a complex of bio-enzymes decomposing urea.
Xylanase a complex of bio-enzymes decomposing plant matter.
Esterase a complex of bio-enzymes decomposing fats.

Used bacterial concentrates are not genetically manipulated or altered they are naturally present in soil and water and carefully selected because they are:

Safe, stable, non-pathogenic and non-toxic.
Capable of growth in the presence of oxygen or without it.
They are a strong producer of enzymes (they do not contain salmonella or listeria).

Main advantages of using bacterial preparations:

Liquidation of vegetable and animal fats.
Significant reduction of contamination by energetic materials (EMs) and ammonia.
Disposal of solid and dissolved substances in tanks.
Destruction of nitrates of any type.
They greatly reduce odours.

From a microbiological point of view, bacterial preparations are harmless.
They do not contain coliform bacteria, faecal coliform bacteria, enterococci, fungi and yeasts.

One gram contains approximately:

12,4 . 109 cultivable bacteria
9,6 . 109 mesophilic bacteria
9,8 . 109 sporulating bacteria

72% of bacteria have growth potential in anaerobic conditions. Another important feature is the use of carbohydrates (carbon), a group consisting of a wide variety of compounds that are generally known as "sugars".

Characteristic of bacterial enzymatic mixtures


Bacteria are the most widespread group of organisms in the world. They can be found in soil, water, air, on the surface and within the multicellular organisms. Some species, according to the research, are able to survive in space, in vacuum, or at temperature of -270 degrees Celsius.

In their lifecycle, so-called sporulating bacteria create spores (a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions). Some bacteria (clostridia, bacilli) are able to create endospores (a dormant, tough, and non-reproductive structure), which are created inside of the cell. In endospores, bacteria survive tough conditions, and later, under more favourable circumstances, they are able to sprout in vegetative cells.

Bacteria produce enzymes.


Enzymes are proteins, whether simple or compound, which act as catalysts for chemical reactions, while determining the nature and speed of these reactions. A summary of all enzyme reactions that convert substances and energies into cells and living organisms is called metabolism.

One of the very important functions of enzymes that directly depends on the life and growth of organisms is the ability of various enzymes to digest and decompose various complex molecules into smaller strata that the organisms are able to digest and thus become the source of energy for their further growth.


By the term bacterial-enzymatic mixture, we mean a concentrate of specially selected spores and endospores enriched by target-grown strains of original soil bacteria that possess certain desired characteristics, such as ability to survive and multiply in a certain pH environment; or the ability to increase the production of a particular enzyme. The mixtures used by ProEco are characterized by a significant concentration of microorganisms. In some concentrates there are up to 5 x 10 CFU /per one gram of mixture (CFU - colony forming unit)

Individual mixtures and their combinations form the basis for the final commercially available products, which are depending on their composition directed to the practical solution of certain particular problems, such as degradation of fats in a certain environment; homogenization of manure; reduction of ammonia and odour level in general; wastewater treatment; excrement degradation; or leakage of pipelines

After putting the mixture into an aqueous environment containing organic waste, spores begin to come to life in a short time; they produce specific enzymes, and feed with the organic waste present in the water. Applied microorganisms grow exponentially (they are doubled every 20-30 minutes). The by-products of this bacterial enzyme activity are H2O and CO2.

It is important to emphasize that all strains of bacteria used in our bacterial-enzymatic mixtures are non-pathogenic and that they are always and exclusively related to strains of naturally occurring soil bacteria that have been selected for a certain specific ability. These strains are by no means genetically altered or modified.

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